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. 2018 Mar 26;177(1):255–270. doi: 10.1104/pp.17.01759

Figure 7.

Figure 7.

The rmd-1 mutant has alterations in pollen tube cell wall composition. A, Wild-type (WT) and rmd-1 pollen grains were germinated for 15 min, fixed, and labeled with the monoclonal antibody JIM5. Secondary antibodies were conjugated with FITC. BF, Bright field. Bars = 10 μm. B, Quantification analysis of JIM5 labeling in apical and shank zones of wild-type and rmd-1 pollen tubes as mentioned in A (n > 24 of each genotype). a.u., Arbitrary units; FI, fluorescence intensity. C, Wild-type and rmd-1 pollen grains were germinated for 15 min, fixed, and labeled with the monoclonal antibody JIM7. Secondary antibodies were conjugated with FITC. Bars = 10 μm. D, Quantification analysis of JIM7 labeling in apical and shank zones of wild-type and rmd-1 pollen tubes as mentioned in C (n > 20 of each). All error bars represent sd of at least three independent experiments. Student’s t test: **, P < 0.01.