Stages of unfertilized seeds and early fertilized seeds encompassing the three phases of endosperm development used in the RT-qPCR assays. Cleared ovules or seeds at 3 DAE (A and H), 0 DAP (B and I), 1 DAP (C and J), 2 DAP (D and K), 3 DAP (E and L), 4 DAP (F and M), and 5 DAP (G and N) from Col-0 (A–G) and mea-3;swn-3 mutant (H–N) plants are shown. The embryo is false colored in yellow. The embryo sac or the part of the embryo sac excluding the embryo is false colored in blue. Bars = 50 µm.