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. 2018 Mar 9;177(1):285–299. doi: 10.1104/pp.17.00534

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Figure 4. — Differential imprinted expression patterns of C2 type I MADS-box genes. The allele-specific expression of 14 C2 genes and control genes was analyzed in reciprocal crosses between Col-0 and Cvi-0 or Ler by single-base extension with fluorescently labeled ddNTPs (SBE) based on SNPs. Orange denotes maternal allele expression frequency, and blue denotes paternal allele expression frequency. In cross descriptions, the maternal parent is denoted first. FIS2 represents a MEG, and AGL62 represents a biallelically expressed gene. Three independent biological replicates were used. The threshold for determining MEGs and PEGs was set at and (where m denotes maternal transcripts and p denotes paternal transcripts measured by SBE) in both reciprocal crosses, as described previously (Hsieh et al., 2011). P values calculated using Student’s t test are provided in Supplemental Table S5.

Inline graphic — Differential imprinted expression patterns of C2 type I MADS-box genes. The allele-specific expression of 14 C2 genes and control genes was analyzed in reciprocal crosses between Col-0 and Cvi-0 or Ler by single-base extension with fluorescently labeled ddNTPs (SBE) based on SNPs. Orange denotes maternal allele expression frequency, and blue denotes paternal allele expression frequency. In cross descriptions, the maternal parent is denoted first. FIS2 represents a MEG, and AGL62 represents a biallelically expressed gene. Three independent biological replicates were used. The threshold for determining MEGs and PEGs was set at and (where m denotes maternal transcripts and p denotes paternal transcripts measured by SBE) in both reciprocal crosses, as described previously (Hsieh et al., 2011). P values calculated using Student’s t test are provided in Supplemental Table S5.