Figure 3.

Cellulose content of the plants containing an Asp-to-Asn mutation in the TED motif of secondary wall CESAs in alternative cesa backgrounds. A, Secondary wall CESA T-DNA null mutants (cesa8^irx1-4, cesa7^irx3-6, and cesa4^irx5-6) were transformed with TED Asp-to-Asn mutants (CESA8^D683N, CESA7^D726N, and CESA4^D748N, respectively). Transformation of wild-type (WT) genes was not performed for this set of mutants. B, cesa7^irx3-1 and cesa4^irx5-2 were transformed with either the wild-type genes (CESA7 and CESA4, respectively) or TED Asp-to-Asn mutants (CESA7^D726N and CESA4^D748N, respectively) and compared with the cesa8^irx1-1 mutant. For CESA8, Ler wild-type measurements have been duplicated in the middle graph in order to make easy comparisons. C, cesa8^irx1-7, cesa7^irx3-6, and cesa4^irx5-4 were backcrossed into Ler five times to create cesa8^irx1-7L, cesa7^irx3-6L, and cesa4^irx5-4L, respectively. These were then transformed with either the wild-type genes (CESA8, CESA7, and CESA4, respectively) or TED Asp-to-Asn mutants (CESA8^D683N, CESA7^D726N, and CESA4^D748N, respectively). N refers to the number of individual T1 transformants analyzed. Cellulose content is expressed as a percentage of the wild-type value. Error bars indicate se. Significance levels from univariate ANOVA are indicated for comparison between the genotype and the background mutant (white asterisks inside each bar) or between particular genotypes (black asterisks and lines above the bars): ***, significant at 0.001; **, significant at 0.01; and *, significant at 0.05.