Figure 2.

ALY proteins interact with UAP56 in yeast two-hybrid assays and FRET analyses. A, Yeast two-hybrid assays with cells harboring the indicated constructs grown on DDO (left) or QDO (right) medium. B, Protein interactions analyzed by FRET-APB. N. benthamiana leaves were coinfiltrated with vectors expressing donor (eGFP, ALY1, and ALY3; green)/acceptor (mCherry and UAP56; red) combinations as indicated, alongside positive and negative controls. Transiently transformed cells were analyzed using two biological replicates by FRET-APB. Mean FRET-APB efficiencies (±sd, eight analyzed nuclei each) are shown. ***, Statistically significant difference by Student’s t test (P < 0.001).