Figure 5.

Localization of ALY-GFP fusion proteins in pollen grains and ovules. A, Mature pollen grains. Closed and open arrowheads indicate sperm cell nuclei and vegetative nuclei, respectively. DNA is counterstained with DAPI (blue). GFP fluorescence (left images) and the corresponding bright-field images, merged with fluorescent signals (right images), are shown. B, Mature unfertilized ovules. GFP fluorescence (left images) and the corresponding bright-field images, merged with GFP signals (right images), are shown. Insets depict ALY-GFP signals in the nuclei of female gametophytic cells. Closed and open arrowheads and asterisks indicate synergid nuclei, egg cell nuclei, and central cell nuclei, respectively. Bars = 5 µm (A) and 25 µm (B).