Figure 7.

4xaly plants produce a number of flowers with altered morphology and abnormal ovules. A to F, Flower morphology. A, Col-0 flower depicting normal petal number. B, 4xaly flower depicting altered petal number. C, Normal 4xaly flower as observed in the majority of cases. D, Col-0 flower depicting normal trichome number on sepals and stamen filament length (closed arrowheads). E and F, Abnormal 4xaly flower depicting increased trichome number on sepals (open arrowheads) and reduced stamen filament length (closed arrowheads). G to K, Differential contrast microscopy of cleared ovules. G, Col-0 ovule depicting normal morphology. H, 4xaly ovule depicting normal morphology. I, 4xaly ovule depicting arrested female gametophyte development. J, 4xaly ovule depicting collapsed female gametophyte. K, 4xaly ovule depicting abnormal integument development. Closed and open arrowheads and asterisks indicate synergid cell nuclei, egg cell nuclei, and central cell nuclei, respectively. FG, Female gametophyte; II, inner integuments; OI, outer integuments. L and M, Morphology of self-pollinated siliques of the indicated genotypes (pistil × pollen). N to Q, Morphology of cross-pollinated siliques of the indicated genotypes (pistil × pollen). Open arrowheads in M indicate gaps with undeveloped seeds in a 4xaly silique. Bars = 0.2 mm (A–F), 20 µm (G–K), and 2 mm (L–Q).