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. 2018 Mar 26;177(1):300–310. doi: 10.1104/pp.18.00218

Figure 5.

Figure 5.

Mitochondrial genome instability in RECX KO and OEX strains. A, qPCR analyses of products from recombination between mitochondrial SDRs in RECX KO and OEX strains. The relative copy numbers of products from recombination between repeats (R2–R23) are normalized against nuclear actin. Data are means of three technical replicates ± sd. Asterisks indicate significant differences (P < 0.05, Student’s t test). B, DNA gel-blot analysis of the configuration at the mitochondrial nad7 locus. SacII-digested total DNAs from wild-type (WT), RECX KO, and RECX OEX strains, and the RECA1 KO mutant, were probed with labeled nad7 DNA. The asterisk denotes products from recombination between nad9-nad7 repeats comprising R1 and R14. C, qPCR analysis of gene dosage at three mitochondrial loci. The relative copy numbers of mitochondrial rps4, nad6, and rpl2 are normalized against nuclear actin. Data are means of three technical replicates ± sd.