Figure 7.

RECX interacts with RECA1 and RECA2. Protein-protein interactions were investigated by yeast two-hybrid assay with HIS3 as a reporter gene. Yeast strains harboring genes fused to the activation domain (AD) and the DNA-binding domain (BD), or individual AD/BD gene fusions alongside empty vector controls, were spotted onto standard medium lacking Leu and Trp (-LW) or selective medium lacking Leu, Trp, and His (-LWH) following 10-fold serial dilution. 3-Amino-1,2,4-triazole (3-AT) was added to -LWH medium at two different concentrations (1 and 5 mm).