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. 2018 Mar 9;177(1):216–225. doi: 10.1104/pp.17.01722

Figure 2.

Figure 2.

Functional loss of AP‐3 reduces pollen tube growth in vitro. A to E, In vitro pollen tubes from the wild type (WT; A), ap-3δ (B), ap-3β (C), ap-3µ (D), and ap-3σ (E) at 4 h after germination. Two representative pollen tubes from each genotype are highlighted in pink. Bars = 50 µm. F to H, Germination ratio (F), length (G), and apical width (H) of pollen tubes after 4 h of incubation in germination medium. Results shown are means ± sd (n = 300 for F, 100 for G, and 60 for H). All mutants are not significantly different from the wild type in the germination ratio (one-way ANOVA, Dunnett’s multiple comparison test, P > 0.05). Each mutant is significantly different from the wild type in the length or width of pollen tubes, as indicated by asterisks (one-way ANOVA, Dunnett’s multiple comparison test, P < 0.05).