Figure 4.

Vacuolar organization is compromised in ap‐3 pollen tubes. Growing wild-type (A) and ap-3δ (B) pollen tubes expressing VHA-a3-YFP are shown. From top to bottom: the YFP channel image at the midoptical plane; merge of the YFP and transmission channels; and 3D surface rendering of confocal laser scanning fluorescence micrography (CLSM) images. Dotted lines illustrate the silhouettes of the pollen tubes. Arrowheads point at the ring-shaped structures labeled with VHA-a3 and associated with the tubular vacuoles. Over 30 pollen tubes for each genotype were examined with similar results. Bars = 10 μm.