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. 2018 Mar 9;177(1):216–225. doi: 10.1104/pp.17.01722

Figure 5.

Figure 5.

The tonoplast association of PAT10 and CBL2 relies on AP‐3 in pollen tubes. A and B, CLSM images of pollen tubes expressing PAT10g-GFP;VHA-a3-RFP in the wild type (WT; A) and in ap-3δ (B). C, CLSM images of a pollen tube expressing PAT10g-GFP in the complemented AP-3δ-RFP;ap-3δ. D, Pollen tube length. Results shown are means ± sd (n = 100). Means with different letters indicate significant differences of pollen tube length (one-way ANOVA, Dunnett’s multiple comparison test, P < 0.05). E and F, Pollen tubes stably transformed with ProUBQ10:CBL2-RFP in the wild type (E) and in ap-3δ (F). G and I, Pollen tubes stably transformed with ProUBQ10:CBL2-RFP;PAT10g-GFP in ap-3β (G), ap-3µ (H), and ap-3σ (I). G/T or R/T indicates merge of the GFP or RFP channel and the transmission channel; G/R/T indicates merge of the GFP channel, the RFP channel, and the transmission channel. Over 30 pollen tubes for each genotype were examined with similar results for data shown in A to C and E to I. Bars = 10 μm.