Table I. AP-3 loss-of-function resulted in defective male transmission.
| Parents (Female × Male) | F1 Progeny |
||
|---|---|---|---|
| Genotypea | Expected Ratio | Observed Ratio | |
| ap-3δ +/− × wild type | ap-3δ +/+:+/− | 1:1 | 74:68 |
| Wild type × ap-3δ +/− | ap-3δ +/+:+/− | 1:1 | 87:7b |
| ap-3δ +/− × ap-3δ +/− | ap-3δ +/+:+/−:−/− | 1:2:1 | 13:20:2c |
| ap-3β +/− × wild type | R:S | 1:1 | 94:87 |
| Wild type × ap-3β +/− | R:S | 1:1 | 11:159b |
| ap-3β +/− × ap-3β +/− | R:S | 3:1 | 369:211d |
| ap-3μ +/− × wild type | ap-3μ +/+:+/− | 1:1 | 99:107 |
| Wild type × ap-3μ +/− | ap-3μ +/+:+/− | 1:1 | 170:8b |
| ap-3μ +/− × ap-3μ +/− | ap-3μ +/+:+/−:−/− | 1:2:1 | 42:38:16c |
| ap-3σ +/− × wild type | R:S | 1:1 | 125:110 |
| Wild type × ap-3σ +/− | R:S | 1:1 | 39:56b |
| ap-3σ +/− × ap-3σ +/− | R:S | 3:1 | 409:150d |
a
R for Basta resistant and S for Basta sensitive. Basta sensitivity was used to distinguish the mutant copy of ap-3β or ap-3σ, resistant to Basta salt, from its wild-type copy.
b
Significantly different from the segregation ratio 1:1 (χ2, P < 0.01).
c
Significantly different from the segregation ratio 1:2:1 (χ2, P < 0.01).
d
Significantly different from the segregation ratio 3:1 (χ2, P < 0.01).