Figure 6.

Measurement of Ca²⁺ fluxes in response to light-to-dark transition. Ca²⁺ assays were conducted in Arabidopsis seedlings stably expressing YA chimeras in different subcellular locations: stroma (A; blue trace), thylakoid lumen (B; green trace), thylakoid membrane (C; violet trace), cytosol (D; gray trace), and cytosolic surface of the plastid outer envelope (E; brown trace). Data are presented as means ± se (black shading) of 15 traces obtained from 15 different plants derived from five independent growth replicates. In all graphs, the lights-off stimulus starts at time 0.