Figure 7.
Purification, identification, and enzymatic activity of FaSAMDC protein. A, Purification of the 66-kD recombinant FaSAMDC protein. B, Immunoblot identification of the recombinant FaSAMDC. C, Measurement of the affinity between SAM and the purified FaSAMDC protein using isothermal titration calorimetry. A typical and specific saturation curve with stoichiometry (N) of 1:1 was obtained, suggesting that one SAM molecule could bind per purified protein molecule with a dissociation constant of 1.7 × 10−3 m.