Figure 3.
Signaling events in response to sunflecks perceived by UVR8. A and B, Nuclear fluorescence of YFP-COP1 in the UVR8 (cop1-4/Pro35S:YFP-COP1) and uvr8 mutant background (cop1-4 uvr8-6/Pro35S:YFP-COP1) measured by confocal microscopy. C, UVR8 enhances HY5 expression under sunflecks. Seedlings were grown under deep shade for 7 d, transferred to sunfleck conditions at midday, and harvested 2 h later. Data are means ± se of 30 (A) or 7 (C) biological replicates and the significance (regression analysis) of the interaction between UVR8 and light condition is indicated (A and C). Representative confocal images are shown in B.