Effects of Fe availability on the growth of Col-0, f6’h1-1, s8h-3, s8h-4, S8H Ox1, and S8H Ox2 plants. A, Phenotypes of plants grown for 14 d on either ES or navFe media. Representative images of five independent experiments are shown. Bar = 1 cm. B, Shoot fresh weight. C, Shoot Fe content. D, Chlorophyll content. Each bar represents the mean ± se of five independent experiments. Statistical testing was carried out using Student’s t test. Asterisks indicate significant differences from the wild type in each treatment: *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; ****, P ≤ 0.0001.