Figure 5.

Heterologous expression of AtBBX21 increases stomatal aperture in potato leaves. A, Stomatal density and stomatal index estimated as the number of abaxial stomata/adaxial stomata of nontransformed (Spunta) and transgenic (CH2) potato leaves (n = 3). B, Stomatal aperture (n = 3). C, Representative photographs of stomata in nontransformed and BBX21-overexpressing lines. S, Stomata; O, opercule. Bar = 10 µm. n = number of biological replicates. Mean values are shown with error bars indicating se. Data were analyzed by Student’s t tests, and asterisks indicate significant difference between nontransformed and transgenic lines (*P < 0.05 and ***P < 0.001; NS, not significant).