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. 2018 Mar 15;177(1):168–180. doi: 10.1104/pp.18.00023

Figure 4.

Figure 4.

Identification of GA2oxA9 as a candidate gene for Rht18. A, Chromosome 6A from Icaro and overgrowth mutants isolated and Illumina-sequenced at greater than 80× coverage. B, Icaro Illumina reads de novo assembled and sequence of the overgrowth mutants mapped back to the assembly. C, Contig (22631_1) was identified which contained gene TraesCS6A01G221900 (GA2oxA9). D, Gene model of GA2oxA9 showing three exons and five independent mutations (G/A or C/T transitions) in durum Icaro background. Additional mutations were found in eight Halberd overgrowth mutants (Hal-M47, Hal-M75, Hal-M13, Hal-M46, Hal-M30, Hal-M10, Hal-M1, and Hal-M26). E, Predicted amino acid sequence of GA2oxA9 protein showing single amino acid substitutions in durum and hexaploid wheat mutants except in M24 and Hal-M75 where mutations resulted in early termination codons and Hal-M1 and Hal-M10 where mutations resulted changing spliceosome recognition site. Predicted functional domains include nonhaem dioxygenase (DIOX_N) domain and 2-oxoglutarate and Fe(II)-dependent oxygenase [2OGFe(II)_Oxy] domain.