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Specifications of mRNA Species, Primer Sequences, PCR Annealing Temperature, and Product Size.
| mRNA | 5′ Primer | 3′ Primer | °C | Size (bp) |
|---|---|---|---|---|
| 28S | GTGCAGATCTTGGTGGTAGTAGC | AGAGCCAATCCTTATCCCGAAGTT | 58 | 552 |
| Collagen I | GTGAAAATGGAGCTCCTGGT | GAGCACCATTGGCACCTTTA | 59 | 310 |
| Collagen III | CTGAACTCAAGAGTGGAGAA | TCAGCCTTGAATTCACCTTC | 59 | 399 |
Abbreviations: bp, base pair; mRNA, messenger RNA; PCR, polymerase chain reaction.
