Figure 2.

Fenretinide decreases proliferating cell nuclear antigen (PCNA) and increases cleaved poly (ADP-ribose) polymerases (PARP) protein levels: A, OSIS cells were treated with vehicle (dimethyl sulfoxide [DMSO]) and fenretinide for 12 days. B, OSIS cells were treated and increasing concentration of fenretinide (vehicle, 5, 10, 15, 20 µmol/L). Western blot was performed to check PCNA, PARP (total and cleaved), and actin protein levels. (L = ladder, UT = DMSO control, CTL= tumor necrosis factor α [TNF-α], apoptosis control). Data are representative of experimental replicates from 4 patients (*p < 0.05).