Figure 1.

B-cell CLL/lymphoma 6 (BCL6) expression at different states. A, Quantitative RT polymerase chain reaction (RT-PCR) levels of BCL6 messenger RNA (mRNA) in whole normal endometrium evaluated each cycle phase, determined by cycle day and urine luteinizing hormone (LH) detection. Mid-secretory levels of BCL6 were significantly higher compared to proliferative phase (P = .02, Kruskal-Wallis with Dunn post hoc test). B, HSCORE values based on immunohistochemical expression of BCL6 across the menstrual cycle in normal women. No statistical difference was found among groups (Kruskal-Wallis). C, Quantitative RT-PCR levels of BCL6 mRNA in separated stromal and epithelium cells from whole endometrial tissue. A higher level of expression was found in the epithelial compartment (P = .0005, Mann-Whitney U test). D, Quantitative RT-PCR levels of BCL6 mRNA obtained from proliferative and secretory phase in women with and without endometriosis. Significant difference was found between secretory phases of both groups and between proliferative and secretory groups of endometriosis (P = .001 and 001, respectively—Kruskal-Wallis with Dunn post hoc test). E, Receiving–operator characteristic (ROC) analysis comparing laparoscopically proven endometriosis to fertile women without endometriosis at the time of laparoscopy. The dotted line represents the ROC-determined HSCORE cutoff of 1.4. F, Immunohistochemical expression (HSCORE) of BCL6 between fertile controls (Fertile) and fertile women without endometriosis at laparoscopy (L/S Controls) compared to those with endometriosis at laparoscopy (L/S E’osis; P < .0001, Mann-Whitney). A further comparison was made between immunohistochemical expression (HSCORE) of BCL6 between women with unexplained infertility (UI) and both fertile control groups (P < .0001, Mann-Whitney U test). All boxes in each graph represent the median levels of mRNA of expression or HSCORE.