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. 2016 Mar 31;23(10):1314–1325. doi: 10.1177/1933719116638186

Figure 4.

Figure 4.

Overexpression of EZH2 using adenoviral vector decreases MSH2 expression in HuLM cells. A, EZH2 was overexpressed in HuLM cells infected with adenovirus containing EZH2 as compared to cells infected with adenovirus containing GFP. Levels of epigenetic mark H3K27me3 were also increased in HuLM cells infected with virus containing EZH2. B, EZH2 was found in both cytoplasm and nucleus; however, higher expression of EZH2 was observed in the nucleus as compared to the cytoplasm. C, MSH2 RNA expression was downregulated in HuLM cells overexpressed with EZH2 as compared to GFP control. D, Cell cycle regulatory gene p27 is downregulated in EZH2-overexpressed cells as compared to GFP control cells. *P < .05 compared with the control. Total cell lysates (E), nucleus (F), and cytoplasm (G) from HuLM cells infected with Ad-EZH2 or Ad-GFP were analyzed by Western blot using Anti-MSH2 antibody. Western blot with anti–β-actin antibody was used as the loading control. MSH2 protein bands were quantified and normalized to β-actin, and relative values were used to generate data graphs (right panels).