Figure 5.

DZNep treatment restores expression levels of MSH2 through epigenetic mark H3K27me3. A, Location of regions analyzed by chromatin immunoprecipitation (ChIP)/polymerase chain reaction (PCR) along human MSH2 promoter. The position of the transcriptional start site is designated as +1. Short horizontal lines indicate regions analyzed by ChIP/PCR. B, ChIP/quantitative PCR (q-PCR) was performed with anti-H3K27me3 antibody in the distal promoter region of MSH2 in human fibroid primary cells in the presence or absence of DZNep. C, ChIP/q-PCR was performed with anti-H3K27me3 antibody in the proximal promoter region of MSH2 in human fibroid primary cells in the presence or absence of DZNep. *P < .05 compared with the control.