Figure 4.

Sw.71 cells treated with 100 IU human chorionic gonadotropin (hCG) have increased attachment to endometrial epithelial cells compared to nontreated controls. Sw.71 cells constitutively expressing green fluorescent protein (GFP) were treated with 100 IU hCG for 24 hours and co-cultured with epithelial carcinoma cell 1 (ECC-1) cells for 1 hour. Once unattached cells were removed, the GFP-positive cells were quantified; one representative experiment is shown (A). Treatment with hCG increased Sw.71 attachment to the ECC-1 monolayer compared to nontreated Sw.71 controls (n = 5, P = .0006; A, B).