Table 1.
Primer Sequences Used for Quantitative Real-Time PCR for Both the Hyper- and Hypomethylated Genes Selected From the Array.
| Gene Name | Forward Primer | Reverse Primer |
|---|---|---|
| MGMT | GCAATGAGAGGCAATCCTGT | TACACGTGTGTGTCGCTCAA |
| DUSP22 | GTGCTGCCAAAAAGAAAAGC | GGATCCTTACAGGCATCCAA |
| CDCA2 | TTTGAAGCACCTGCCTTTCT | CAAGATTCTCCCCCTTGTCA |
| ID2 | CGTGAGGTCCGTTAGGAAAA | ATAGTGGGATGCGAGTCCAG |
| RBBP7 | TCCCAATGATGATGCACAGT | AGGATTCTGCGGCATGTAAC |
| TNFRSF1B | GGATGAAGCCCAGTTAACCA | GCAGAGGCTTTCCACAACTC |
| BMPR1B | AAAGGTCGCTATGGGGAAGT | GCAGCAATGAAACCCAAAAT |
| ZNF681 | TGGTCTCAGCTCACTGCAAC | GGGGTCAGGAGTTCAAGACA |
| IGSF21 | AAGCGAGAGGACCTGGTGTA | GCCATGACGTTGAGGAAGAT |
| TP73 | GGCAGGTCAGCTCACATCT | GAGTGGATGTTTGTGCGTTG |
Abbreviations: MGMT, O-6-methylguanine-DNA methyltransferase; DUSP22, dual specificity phosphatase 22; CDCA2, cell division cycle associated 2; ID2, inhibitor of DNA binding 2; RBBP7, retinoblastoma binding protein 7; BMPR1B, bone morphogenetic protein receptor, type IB; TNFRSF1B, tumor necrosis factor receptor 1B; ZNF681, zinc finger protein receptor 681; IGSF21, immunoglobin superfamily, member 21; TP73, tumor protein 73.