Figure 4.

Bentamapimod (AS-01) causes reduction in the expression of genes previously associated with endometriosis in endometriotic-like lesions established with biopsies from patients with disease. TaqMan quantitative polymerase chain reaction was performed on lesions from each treatment group within an experiment. The lesions from 4 independent experiments were analyzed, and the mean responses are represented. Gene expression analysis was conducted on a TaqMan low-density array. The responses are grouped according to proinflammatory cytokines (A), matrix proteins (B), and steroidogenic pathway (C). The results are normalized to hypoxanthine phosphoribosyltransferase 1 (HPRT1) expression, and error bars represent standard deviation determind by analysis of variance (ANOVA) followed by Tukey test. Significance (P < .05) is represented by asterisks relative to control groups receiving estrogen alone (dotted line at 1.0).