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. 2018 Apr 30;51(4):188–193. doi: 10.5483/BMBRep.2018.51.4.209

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Copyright © 2018 by the The Korean Society for Biochemistry and Molecular Biology

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3 — Effects of 5-HT2BR inhibitor (SB) on G2/M progression induced by CaS or 5-HT. (A) HaCaT cells synchronized at G2/M arrest by nocodazole were pretreated with 10 μM SB215505 before treatment with CaS and 5-HT for 24 h. These cells were then fixed and stained with PI and analyzed by FACS. (B–D) SB215505 treatment induced delay of G1 release (B) and increase G2/M population (D) without causing significant change in S population (C). Filled symbols represent CaS or 5-HT treatment only. Empty symbols represent cells pre-treated with SB215505 before 5-HT or CaS treatment. (E) Cells were pre-treated with SB215505 (10 μM) to inhibit 5-HT2BR for 30 min before treatment with 10 μM of 5-HT or CaS. After 6 h of treatment with 5-HT or CaS, cell lysates were collected and cyclin B protein levels were measured by western blotting.