Cell type | Cells seeded per ring | Ring diameter | Culture Duration | Functional analysis performed |
Rat SMC | 0.5, 1, or 3 x 106 | 2, 4, or 12 mm | 3 days | Current study; N/A |
iPSC derived SMC 11 | 0.6 x 106 | 2 mm | 17 days | Contractility testing, uniaxial tensile test, histological analysis |
Human SMC 22 | 0.4 x 106 | 2 mm | 2, 7, or 14 days | Uniaxial tensile test, histological analysis |
Human MSC 14 | 0.4 x 106 | 2 mm | 21 days | Uniaxial tensile test, histological analysis, ring fusion and tube compression testing, differentiation into cartilage tissue |