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. 2018 Apr 2;(134):56618. doi: 10.3791/56618
Cell type Cells seeded per ring Ring diameter Culture Duration Functional analysis performed
Rat SMC 0.5, 1, or 3 x 106 2, 4, or 12 mm 3 days Current study; N/A
iPSC derived SMC 11 0.6 x 106 2 mm 17 days Contractility testing, uniaxial tensile test, histological analysis
Human SMC 22 0.4 x 106 2 mm 2, 7, or 14 days Uniaxial tensile test, histological analysis
Human MSC 14 0.4 x 106 2 mm 21 days Uniaxial tensile test, histological analysis, ring fusion and tube compression testing, differentiation into cartilage tissue