Figure 6.

Malto-oligosaccharide incorporation assays. Amylopectin (2.5 mg, ▪; 1 mg, •; 0.25 mg, ▴) was incubated with increasing concentrations of radiolabeled malto-oligosaccharides produced by debranching radioactive C. reinhardtii amylopectin. The y axis represents two different scales giving net incorporation of oligosaccharides within the polysaccharide. An enzyme activity corresponding to 2 nmol of Glc produced from maltotriose per minute was used in these experiments. Incorporation was strictly proportional to enzyme activity for activities ranging between 0.5 and 20 nmol of Glc produced from maltotriose per minute. The label was confined to the outer chains of amylopectin, since all of the radioactive material was liberated upon treatment with β-amylase.