Figure 1.

UV-inactivation abrogates replication of MV-NIS. MV-NIS was inactivated by 0.5 Joule/cm² UV-C light for 0.5 h.

Notes: (A) UV-inactivated MV-NIS is not oncolytic in Vero cells. Vero cells were incubated with dilutions of active MV-NIS, UV-inactivated MV-NIS or buffer and a TCID₅₀ assay was performed. Syncytia formation was documented by using crystal violet staining after 6 d. (B) UV-inactivated MV-NIS does not affect viability of Jurkat cells. Jurkat cells were incubated with buffer (negative control), MV-NIS (positive control) or UV-inactivated MV-NIS at indicated MOIs in the presence or absence of 2% measles immune serum. Cell viability was determined after 72 h by flow cytometry using forward scatter/side scatter analysis. Results are means of triplicates. Statistical analysis was performed using a two-tailed unpaired Student’s t-test. *p < 0.05; ****p < 0.0001.

Abbreviations: UV, ultraviolet; UV-C, short-wavelength ultraviolet light; MV-NIS, human sodium iodide symporter measles virus; MOI, multiplicity of infection; ns, not significant; TCID₅₀, tissue culture infective dose 50.