| Component | Volume |
| 2x dPCR supermix for probes (no 2’-deoxyuridine 5’-triphosphate) | 10 µL |
| 20x variant target primers/probes set (450 nmol/L primers, 250 nmol/L FAM probe) | 1 µL |
| 20x control target primers/probe set (450 nmol/L primers, 250 nmol/L HEX probe) | 1 µL |
| nuclease-free water | 1 µL |
| cDNA | 7 µL |
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