Fig. 6. (A and B) Confocal microscopic tracking of the mitochondrial morphology in real-time using 2 (10 μM) and 4 (10 μM) over a period of 8 h. 2: λex = 405 nm; λem = 630 ± 20 nm. 4: λex = 405 nm; λem = 560 ± 20 nm. The cell borders are recognizable and outlined with the red curve. Scale bar: 10 μm. (C) Representative TEM images showing the perinuclear clustering of abnormal swollen mitochondria of A549 cells treated with 2 (d–f) and 4 (g–i) at a concentration of 5 μM for 24 h. (b) and (c) are enlarged views of the red zones in (a). (e) and (f) are enlarged views of the red zones in (d). (h) and (i) are enlarged views of the red zones in (g).
