Media Component	Stock Solution	Amount	Storage
DMEM / F-12 Nutrient Mixture "Base Media"	Use as is	2x 500 mL containers	Store at 4 ºC up to manufacturer expiration date
Ultroser-G	20 mL of sterile water in a single, 20 mL bottle of lyophilized Ultroser-G	Entire 20 mL aliquot	Make fresh with each batch. Store powder at 4 ºC up to manufacturer expiration date
Fetal Clone II	Use as is	20 mL	Store at -20 ºC up to manufacturer expiration date
Pen Strep	Use as is	10 mL	Store at -20 ºC up to manufacturer expiration date
Bovine Brain Extract	Use as is	2.48 mL	Store at -20 ºC up to manufacturer expiration date
Transferrin	Use as is	250 µL	Store at -20 ºC up to manufacturer expiration date
Insulin	Use as is	250 µL	Store at -20 ºC up to manufacturer expiration date
Ethanolamine	Use as is	15 µL	Store at room temperature up to manufacturer expiration date
Epinephrine	2.75 mg in 1 ml of sterile water	Entire 1 mL aliquot	Make fresh with each batch. Store powder at 4 ºC up to manufacturer expiration date
Triiodothyronine	8.4 mg in 50 µL of DMSO	Entire 50 µL aliquot	Make fresh with each batch. Store powder at -20 ºC up to manufacturer expiration date
Hydrocortisone	7.24 mg in 1 mL of ethanol	1 µL	Store stock at -20 ºC up to six months
Phsophoryletheanolamine	35.25 mg in 1 mL of sterile water	1 µL	Store stock at -20 ºC up to six months
Retinoic Acid	3 mg in 1 mL of DMSO	1 µL	Store stock at -20 ºC up to six months