Media Component	Stock Solution	Amount	Storage
Expansion Media
DMEM / F-12 Nutrient Mixture "Base Media"	Use as is	2x 500 mL containers	Store at 4 ºC up to manufacturer expiration date
Fetal Bovine Serum	Use as is	50 mL	Store at -20 ºC up to manufacturer expiration date
Cholera Toxin	10 mg in 1 mL of sterile water	1 µL	Store stock at -20 ºC up to six months
Epidermal Growth Factor	500 µg in 1 mL of sterile water	20 µL	Store stock at -20 ºC up to six months
Hydrocortisone	0.4 mg in 400 µL of sterile water	Entire 400 µL aliquot	Make fresh with each batch. Store powder at room temperature up to manufacturer expiration date
Adenine	24 mg in 1 mL of sterile water	Entire 1 mL aliquot	Make fresh with each batch. Store powder at room temperature up to manufacturer expiration date
Y-27632	3.2 mg in 1 mL of sterile water	Entire 1 mL aliquot	Make fresh with each batch. Store powder at -20 ºC up to manufacturer expiration date
Antibiotic Media
Amphotericin B	Use as is	1.2 mL	Store at 4 ºC up to manufacturer expiration date
Ceftazidime	15 mg in 1 mL of sterile water	Entire 1 mL aliquot	Make fresh with each batch. Store powder at -20 ºC up to manufacturer expiration date
Tobramycin	15 mg in 1 mL of sterile water	Entire 1 mL aliquot	Make fresh with each batch. Store powder at -20 ºC up to manufacturer expiration date
Vancomycin	15 mg in 1 mL of sterile water	Entire 1 mL aliquot	Make fresh with each batch. Store powder at -20 ºC up to manufacturer expiration date