Effect of DTT on NO generation from GTN and DEA/NO by C301S/C303S-ALDH2 overexpressing VSMCs. (A) C301S/C303S-ALDH2 overexpressing VSMCs were perfused with 1 μM GTN in the absence (red) and presence (blue) of 1 mM DTT, and the NO concentration was monitored by fluorescence microscopy. See Materials and Methods for further details (n = 3, 13 and 14 traces in the absence and presence of DTT, respectively). (B) C301S/C303S-ALDH2 overexpressing VSMCs were perfused with 10 μM DEA/NO as indicated in the absence (red) and presence (blue) of 1 mM DTT, and the NO concentration was monitored by fluorescence microscopy. See Materials and Methods for further details (n = 3, 31 and 18 traces in the absence and presence of DTT, respectively).