Figure 8.

N-terminal amino acid analysis of U. panicoides PCK polypeptides. PCK was purified from U. panicoides leaves using a method that results in the stepwise cleavage of up to 8 kD from the N terminus of a high proportion of the polypeptide chains (Finnegan and Burnell, 1995). The mixture of cleavage products was subjected to Edman degradation. The amino acid residues released in each cycle are shown in the matrices and are arranged in order of decreasing abundance (at the top of each column is the most abundant residue released). The sequences below the line in each matrix were deduced from the PCK1 and PCK2 cDNAs. The Roman numerals correspond to the sequences previously identified (Finnegan and Burnell, 1995). In the body of each matrix, residues found in both PCK1 and PCK2 sequences are indicated by white boxes, whereas residues found in only the PCK1 or the PCK2 sequence are indicated by shaded and white circles, respectively. This analysis was also performed with the SDS-PAGE-purified 62- and 61-kD PCK degradation products (Finnegan and Burnell, 1995).