Figure 1.
Effects of the initial cell density on TE differentiation. Single zinnia cells were suspended in liquid medium, dispensed into 24-well culture plates at a final volume of 500 μL per well, and incubated at 25°C with shaking at 120 rpm. The TE differentiation frequency for each well was calculated by dividing the number of TEs by the total number of living cells. Data are mean values of three replicates ± sd. □, 1 × 105 cells/mL; •, 5 × 104 cells/mL; ▵, 2.5 × 104 cells/mL; ▪, 1.3 × 104 cells/mL; ○, 6.2 × 103 cells/mL; ▴, 3.1 × 103 cells/mL.