Effects of PSK-α on TE differentiation (A) and cell viability (B). Single zinnia cells were incubated in liquid medium at a density of 2.5 × 104 cells mL−1 in the presence of various concentrations of PSK-α. The TE differentiation frequency for each well was calculated by dividing the number of TEs by the total number of living cells, and cell viability was calculated by dividing the number of living cells by the number of total cells. Data are means of three replicates ± sd. □, 1.0 μm; •, 0.1 μm; ▵, 0.01 μm; ▪, 1.0 nm; ○, 0.1 nm; ▴, control.