Fig 1. MS spectra of the alkaline de-N-glycosylation products of MAB-1-L001.

(A) Products obtained from the reaction in 5% hydroxylamine; (B) products obtained from the reaction in 20% hydroxylamine. Both reactions were executed in hydroxylamine and 200 mM LiOH (final concentration) at 80°C for 1 h. The inserts show enlarged spectra of G0F(oxime and free glycan). G0F: fucosyl agalacto bi-antennary N-linked glycan; G1F: fucosyl monogalacto bi-antennary N-linked glycan. Glycan structures are depicted by using the CFG graphical notation for glycans (http://www.functionalglycomics.org/static/consortium/CFGnomenclature.pdf).