Fig 3. Time course of alkaline de-N-glycosylation with 20% hydroxylamine at 80°C.

(A) HPLC chromatograms of 2-AB-labeled N-linked glycans released by 0.5 h-reaction, 1 h-reaction, 2 h-reaction, and 4 h-reaction. (B) Comparison of glycan recovery. Bar graph indicates sum of all glycan peak areas in the HPLC chromatograms. All chromatograms were obtained by injection of equal volumes of glycan solution prepared from the same quantity of glycoproteins.