Table 3. Assignment of the peaks in HPLC chromatogram of N-glycans of HRP by MALDI-TOF MS.

Glycoprotein	Peak no.	Obsd (m/z)	Calcd (m/z)a	Monosaccharide composition
Horseradish peroxidase	1	1185.49	1185.43	(Man)3 (GlcNAc)2 (Xyl)1
	2	1199.51	1199.44	(Man)3 (GlcNAc)2 (Fuc)1
	3	1331.42	1331.49	(Man)3 (GlcNAc)2 (Fuc)1 (Xyl)1
	4	1347.55	1347.48	(Man)4 (GlcNAc)2 (Xyl)1
	5	1534.25	1534.57	(Man)3 (GlcNAc)3 (Fuc)1 (Xyl)1
	6	1493.28	1493.54	(Man)4 (GlcNAc)2 (Fuc)1 (Xyl)1
	7	982.38	982.35	(Man)3 (GlcNAc)1 (Xyl)1
	8	1167.43	1167.42	(Man)3 (GlcNAc)2 (Xyl)1 -H2O
	9	982.40	982.35	(Man)3 (GlcNAc)1 (Xyl)1
	10b	1329.53	1329.47	(Man)3 (GlcNAc)2 (Fuc)1 (Xyl)1

^a Values were calculated as sodium-adducted ion of 2-AB-labeled glycans.

^b Unreduced 2-AB labeled glycan.