Fig 1. JzTx-V sequence and inhibition of NaV1.7 currents in HEK293 cells.
A. Amino acid sequence and disulfide connectivity of JzTx-V. B. Manual patch clamp traces for control (black) and JzTx-V (0.3 nM; red) channel block at a holding potential of -140 mV (left) or -82 mV (right). Voltage protocols are depicted below the traces. C. JzTx-V (0.3 nM) channel block is partially reversed by high-frequency strong depolarizations following peptide washout. Cells were held at -140 mV and stepped to -10 mV to record NaV1.7 current. Downward arrows indicate time points during which a high frequency protocol (depicted to right of time course; step to +100 mV for 14msec at 10 Hz for 20 sec) was applied.