Table 1. Primers used in the study.
| Gene | Primer sequence | Amplicon size (bp) | Temperature (°C) | Cycle |
|---|---|---|---|---|
| Rat skeletal muscle actin (SkMu Actin) |
5' CACGGCATTATCACCAACTG 3' TCTCACGTTCAGCTGTGGTC |
406 | 58 | 28 |
| Rat ANF |
5' TGCCGGTAGAAGATGAGGTC 3' ATTCACCACCTCTCAGTGGC |
403 | 59 | 32 |
| Rat BNP |
5' TTTTCCTTAATCTGTCGCCG 3' CACTGTGGCAAGTTTGTGCT |
498 | 57 | 32 |
| Rat PP1c |
5' TTAGACGTATTATGCGGCCC 3' CTTAACGCTGTGTTCCCCAT |
549 | 58 | 28 |
| GAPDH |
5' GGGTGTGAACCACGAGAAAT 3' CCACAGTCTTC TGAGTGGCA |
167 | 58 | 25 |
ANF, Atrial Natriuretic Factor; BNP, Brain Natriuretic Peptide; PP1c, Protein Phosphatase 1c; GAPDH, Glyceraldehyde 3-phosphate dehydrogenase