Fig 4. In vitro phosphorylation of SLC11A1 by c-Src.

A fixed amount of GST-SLC11A1 (500ng, a.a.1-a.a.178) was incubated with increasing amounts (50-200ng) of purified Src, as indicated. Phosphorylation levels of protein were analyzed by immunoblotting with anti-phosphotyrosine antibodies (4G10) (top). The same blots were then reprobed with anti-GST antibodies for GST-SLC11A1 fusion protein as a loading control.