Fig 6. Effect of OsWOX4 knockdown on the morphology of leaf primordia.
(A) to (C) Transverse section of the shoot apex in a wild-type plant (A), and mock-treated (B) and DEX-treated (C) transgenic plants carrying pACT1-GVG>OsWOX4:RNAi. Tissues were stained with toluidine blue. M, SAM. (D) to (F) Central region of a P4 leaf primordium. Cells in the central file are indicated by false coloring and asterisks. (G) Number of cells in the central file of a P4 leaf primordium. Data are the mean ± SE (n = 12 [mock]; n = 13 [DEX]). Student’s t-test, *P < 10−4. (H) and (I) In situ localization of DL transcript. The SAM is indicated by the dotted line. Transgenic plants carrying pACT1-GVG>OsWOX4:RNAi were treated as indicated in Fig 2A (A to G) and in Fig 4A (H and I). Bars = 50 μm in (A) to (C), (H) and (I); 10 μm in (D) to (F).