Fig 3. MoMrt4^G69D and MoMrt4^G69E bypass the requirement of MoYvh1.

(A) Comparison of the ΔMoyvh1 and point mutation strains in colony morphology. Guy11, the ΔMoyvh1 mutant, MoMRT4 and mutated alleles complementation strains were cultured at 28°C in darkness for 7 days before being photographed. (B) Spraying assay. Conidial suspensions of each strain were sprayed on rice seedlings. Diseased leaves were photographed 7 days after inoculation. (C) Quantification of lesion type. Quantification of lesion type (0, no lesion; 1, pinhead-sized brown specks; 2, 1.5-mm brown spots; 3, 2–3-mm grey spots with brown margins; 4, many elliptical grey spots longer than 3 mm; 5, coalesced lesions) were measured at 7 days post-inoculation (dpi), counted within an area of 4 cm² and experiments were repeated three times with similar results. Asterisk indicates significant differences at p = 0.01. (D) DAB staining of the excised leaf sheath of rice infected by Guy11, the ΔMoyvh1 mutant, the point mutation strains and complementation strain 36 h after inoculation. Bar = 5 μm.