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. 2018 Feb 26;25(1):1073274818757418. doi: 10.1177/1073274818757418

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© The Author(s) 2018

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 3. — Time course graphical analysis of positively labeled caspase 3, 8, and 9 following VD₃ treatment and/or freezing to −15°C. Number of cells staining positive for each antibody were divided by the total number of Hoechst staining cells to determine the percentage of positively staining cells in each population. Three separate images per well were obtained and data from 3 separate immunofluorescence experiments were combined.