Figure 4. (A) Photomicrographs in a higher original objective (x40) of the different groups (SHAM, OVX, and RLX) and periods (14 and 42 days), in which is possible to observe an increased area of diaminobenzidine-stained cells (brown areas) around the peri-implant bone where the biomarker osteopontin were intense, represented by black arrows, denoting an improvement in the bone mineralization; (B) The chart shows the scores submitted to the Kappa test, in which the index was adjusted to >0.8, representing expression of osteopontin among the groups and in both periods; (C and D) Photomicrographs in a higher original objective (x100) about the 14 and 42 days of peri- implant bone healing from RLX group showing a moderate labeling of the biomarker osteopontin represented by red arrows.
