Figure 4. piRNA-mediated chromatin targeting machinery modulates splicing of the Gypsy retrotransposon in Ovarian Somatic Cells (OSC).

a, Diagram of Gypsy retrotransposon: gray boxes, long terminal repeats; white boxes, coding sequences; red inverted triangle, splicing that generates env mRNA. b, RNA-seq signal (RPM) at the Gypsy splicing donor and acceptor sites in representative control (GFP KD) and piwi KD conditions. Raw data sets from Sienski et al, 2015. Experiments were repeated three times with similar results. c, Percentage of splicing for Gypsy splicing donor and acceptor sites as determined by RNA-seq analysis performed in OSC cells KDs of piRNA machinery components. Bars represent the number of split-reads for the env splicing donor and acceptor junctions normalized to the total number of sense Gypsy reads mapping to the same junction. Results are represented as means. With the exception arx/Gst1, Su(var)205/HP1a, mael, and H1^7,25, experiments were repeated two or more times. Raw data sets from Ohtani et al, 2013⁷; Sienski et al, 2015⁹; Iwasaki et al, 2016²⁵.